Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
نویسندگان
چکیده
BACKGROUND Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry accurately identifies both selected bacteria and bacteria in select clinical situations. It has not been evaluated for routine use in the clinic. METHODS We prospectively analyzed routine MALDI-TOF mass spectrometry identification in parallel with conventional phenotypic identification of bacteria regardless of phylum or source of isolation. Discrepancies were resolved by 16S ribosomal RNA and rpoB gene sequence-based molecular identification. Colonies (4 spots per isolate directly deposited on the MALDI-TOF plate) were analyzed using an Autoflex II Bruker Daltonik mass spectrometer. Peptidic spectra were compared with the Bruker BioTyper database, version 2.0, and the identification score was noted. Delays and costs of identification were measured. RESULTS Of 1660 bacterial isolates analyzed, 95.4% were correctly identified by MALDI-TOF mass spectrometry; 84.1% were identified at the species level, and 11.3% were identified at the genus level. In most cases, absence of identification (2.8% of isolates) and erroneous identification (1.7% of isolates) were due to improper database entries. Accurate MALDI-TOF mass spectrometry identification was significantly correlated with having 10 reference spectra in the database (P=.01). The mean time required for MALDI-TOF mass spectrometry identification of 1 isolate was 6 minutes for an estimated 22%-32% cost of current methods of identification. CONCLUSIONS MALDI-TOF mass spectrometry is a cost-effective, accurate method for routine identification of bacterial isolates in <1 h using a database comprising > or =10 reference spectra per bacterial species and a 1.9 identification score (Brucker system). It may replace Gram staining and biochemical identification in the near future.
منابع مشابه
TITLE: MALDI-TOF Mass Spectrometry for Bacterial Species Identification: A Review of Diagnostic Accuracy and Clinical and Cost-Effectiveness
Bessède E, Angla-Gre M, Delagarde Y, Sep Hieng S, Ménard A, Mégraud F. MALDI Biotyper, experience in routine clinical bacteriology in a university hospital [Abstract]. Clin Microbiol Infect. 2010 Apr 12;16:S523. (Presented at 20th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID); Vienna, Austria; 10 -13 Apr 2010). Bocher S, Abdul-Redha R. Rapid identification using MA...
متن کاملComparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level.
Bacterial identification relies primarily on culture-based methodologies requiring 24 h for isolation and an additional 24 to 48 h for species identification. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is an emerging technology newly applied to the problem of bacterial species identification. We evaluated two MALDI-TOF MS systems with 720 consecu...
متن کاملApplication of MALDI-TOF MS for the Identification of Food Borne Bacteria
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently emerged as a powerful tool for the routine identification of clinical isolates. MALDI-TOF MS based identification of bacteria has been shown to be more rapid, accurate and cost-efficient than conventional phenotypic techniques or molecular methods. Rapid and reliable identification of food-a...
متن کاملRapid Identification of Anaerobic Bacteria Using Matrix Assisted Laser Desorption Ionisation Time-of-flight Mass Spectrometry
متن کامل
Fast and reliable MALDI-TOF MS–based microorganism identification Matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) fingerprinting is a fast and reliable method for the classification and identification of microorganisms, with applications
Matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) fingerprinting is a fast and reliable method for the classification and identification of microorganisms, with applications in clinical diagnostics, environmental and taxonomical research, or food-processing quality control. The BioTyperTM MALDI-TOF MS fingerprinting system allows researchers to perform ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
دوره 49 4 شماره
صفحات -
تاریخ انتشار 2009